Corbett Rotor Gene 6000 User Manual
Corbett Rotor Gene 6000 User Manual Rating: 5,9/10 3665 votes
I needed to switch fom an ABI 7700 system to a Corbett Rotor Gene. I am using SYBR Green jump start master mix (Sigma). I am using a Corbett Robot to pipett a 10 microl reaction. Now, I am not very happy with the dublicates and the amplification curves.
Should the flourescence not be 100? Some of my colleges get curves around 20, that cant be right?
Can make any suggestions what I can do to improve my assay?
Many Thanks.
Corbett Rotor Gene 6000 Manual Pdf
The Rotor-Gene 3000 uses a patented calibration rotor to define absolute temperature inside the tubes as the rotor is spinning. Temperature is measured at three different points across the operating range, any o drift is compensated to within +/-0.2 C. The Rotor-Gene 3000 is the only real-time system available that offers an automated temperature. Rotor Gene Rg 3000 Manual The Rotor-Gene Probe PCR Kit is designed for use with the Rotor-Gene Q and other Rotor-Gene cyclers, providing ultrafast, highly sensitive quantification. REF R-V48(RG,iQ,Mx)-CE, VER 26.03.15–26.03.15 / Page 2 of 19 AMPLIFICATION AND DATA ANALYSIS USING Rotor-Gene 3000/6000 (Corbett instruments” in the Instruction. User-Friendly Software Interface The Rotor-Gene offers the most user-friendly real-time analysis software system currently available. Developed over the past three years based on customer feedback worldwide, the software has been refined to provide an intuitive, Wizard driven interface, enabling highest possible levels of flexibility. Reverse Transcription Protocol (One-step SYBR Green I Dye Detection) PCR Technologies Protocols Table of Contents. Reverse Transcription. Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs. Qiagen/Corbett Rotor-Gene ® 6000. Welcome to the Rotor-Gene 6000 Series Software version 1.7.28. This help manual will instruct the user through run setup and analysis. The Rotor-Gene 6000 real time DNA amplification system is an open chemistry platform allowing the use of any real time chemistry. The software therefore aims to provide support for analysis of. Blackburn) - (User Manual) (Quick Guide) Corbett Rotor Gene 3000 & 6000 Real Time PCR Machines (Room N232. Pathway-focused Gene Expression Analysis - Advanced Real-Time PCR Array respective QIAGEN kit handbook or user manual. Rotor-Gene Q, Rotor-Gene 6000. LightCycler 480. Mx3000p, Mx3005p, Mx4000p.
QUOTE (peetyswoo @ Jul 7 2006, 11:59 PM)
I needed to switch fom an ABI 7700 system to a Corbett Rotor Gene. I am using SYBR Green jump start master mix (Sigma). I am using a Corbett Robot to pipett a 10 microl reaction. Now, I am not very happy with the dublicates and the amplification curves.
[attachment=1287:attachment]
Should the flourescence not be 100? Some of my colleges get curves around 20, that cant be right?
Can make any suggestions what I can do to improve my assay?
Many Thanks.
[attachment=1287:attachment]
Should the flourescence not be 100? Some of my colleges get curves around 20, that cant be right?
Can make any suggestions what I can do to improve my assay?
Many Thanks.
It seems fairly normal compared to the results I get on our Corbett 6000. Did your previous system normalise to 100 fluorescence? Also, your duplicates look awesome, you are only interested in the exponential part of the graph/reaction (right at the bottom).
That's great..
Buick encore shop manual download. By the way, what is the approx. price of the Corbett Rotor Gene 6000?
Thanks
-AmonTobin-
Corbett Rotor Gene 6000 User Manual Free
Something like 34 to 35 thousand USD with 6 channel
That's great..
By the way, what is the approx. price of the Corbett Rotor Gene 6000?
Thanks
By the way, what is the approx. price of the Corbett Rotor Gene 6000?
Thanks